Labquote

Microscope & core hardware. Stand: Axio Examiner.Z1 (upright, motorized Z, manual XY). Motorized XY stage: 120 × 80 mm travel, repeatability ±0.25 µm. Real-time autofocus: Definite Focus 2 for NLO. User I/O box: 8 TTL in / 8 TTL out for external trigger and synchronization. Polarization optics: rotatable polarizer + analyzer set for anisotropy imaging. Illumination: X-Cite 120LED widefield lamp with fast shutter. Imaging modes. Point-scanning confocal (VIS). Multiphoton / second-harmonic generation (NLO). Spectral scanning, lambda-stacking, FCS/FCCS. FRAP & photo-activation with galvanometer ROI. Lasers (factory-integrated). Tunable 690–1040 nm – Coherent Chameleon Ultra II (3.5 W), 140 fs pulses, 80 MHz. 405 nm – 30 mW diode, DNA / DAPI. 445 nm – 50 mW diode, CFP, mCerulean. 488 nm – 20 mW OPSL, GFP, FITC. 561 nm – 25 mW DPSS, Cy3, mCherry. 594 nm – 20 mW HeNe, Texas Red. 633 nm – 10 mW HeNe, Cy5, Alexa 647. Detectors. 32-channel GaAsP QUASAR spectral detector (photon-counting capable, 405–695 nm). 2 × standard PMT detectors (NIR-enhanced). BiG-II non-descanned GaAsP pair for multiphoton (blue-green & green-red bands). Transmitted NDD PMT for SHG / label-free imaging. Filter cubes (widefield side port). Set 49 DAPI – Ex G365 / Em BP 445/50. Set 38 HE GFP – Ex BP 470/40 / Em BP 525/50. Set 43 HE mCherry – Ex BP 550/25 / Em BP 605/70. Set 50 Cy5 – Ex BP 640/30 / Em BP 690/50. Objectives (with matching DIC sliders). Plan-Apochromat 10×, NA 0.45, dry, WD 2 mm. Plan-Apochromat 20×, NA 0.8, dry, WD 0.55 mm. C-Apochromat 40×, NA 1.2, water immersion with correction collar, optimized for live-cell multiphoton. Plan-Apochromat 63×, NA 1.4, oil immersion, highest resolution. Environmental control. PeCon Incubator XL-LSM: 5–40 °C, 0–15 % CO₂, humidity regulation. Temperature Module S: sample cooling to –5 °C / heating to +80 °C (no CO₂). Software (ZEN Black 2012). Spectral Unmixing & Linear Unmix Wizard. FCS & FCCS GaAsP module. FRAP / FLIP bleaching control with recovery analysis. Experiment Designer + Multi-Time / Tile & Position. 3D-VisArt volume-render engine.

N/A

Condition: Power/self tested
Time Frame: 1 Month
Data System: Yes